In this study, following complete Freund's adjuvant- (CFA-) induced unilateral arthritis, genetic ablation (TRPC5 KO mice) or pharmacological blockade of TRPC5 displays augmented weight-bearing asymmetry, enhanced secondary hyperalgesia, and increased cytokine concentrations in the synovial lavage fluid. This evidence concerns the gene TRPC5 and arthritic joint disease.