TNF and glioma: Following a 24 hours incubation of hEGFRvIII:CD3 bi-scFv, PBMCs, and human glioma cells U87-MG-EGFRvIII or control U87-MG cells, we harvested the supernatant and measured the concentration of IFN-ɣ, IL-6, IL-1β, MIP-1α, GM-CSF, and TNFα using the Luminex multiplex platform (figure 3).