Although previous studies based on bulk RNA detection inferred the association between increased Th1 signature and the MSI status in CRC patients,152 scRNA‐seq analysis further illustrated that only CXCL13+BHLHE40+ Th1‐like cluster but not the classical CD4+ Tem cluster in CRC was preferentially enriched in MSI patients and could be accountable for the favorable response of these patients to CPIs. This evidence concerns the gene BHLHE40 and colorectal carcinoma.