To determine the effects of BUB1 in liver cancer cells, the MHCC97-L and YY-8103 cells were transfected with plasmids containing either an empty p23 vector or BUB1 overexpression vectors (Flag-BUB1), whereas shRNA targeting BUB1 was transfected into HepG2 and Huh7 cells. The gene discussed is BUB1; the disease is liver cancer.