Western blot results, using melanoma cell line lysate (SKMel28) as positive control (Fig. 2E – as groupings of blots cropped from different parts of the same gel) show UC-isolated EV populations were positive for CD9, a common EV marker, and ExoQuick-isolated EV populations were positive for CD9, and also TSG-101, and CD63, two other common EV markers. Here, CD9 is linked to melanoma.