To validate whether CLDN4 is involved in ZNF703-mediated HCC invasion and metastasis, we established two stable cell lines: upregulation of CLDN4 expression in HCCLM3-shZNF703 cells and downregulation of CLDN4 expression in SMMC7721-ZNF703 cells. This evidence concerns the gene CLDN4 and hepatocellular carcinoma.