To test the effect exerted by melanoma-specific bcl-2 overexpression on macrophage functions, THP-1 monocytes were differentiated to macrophages (M0) and exposed for 24 hours to CM derived from parental melanoma M14 or A375SM-SC1 cells or their bcl-2 overexpressing counterparts (online supplementary figure S1A, B). The gene discussed is BCL2; the disease is melanoma.