To test whether IFN-λ restricts RABV replication in vitro, NA or Vero cells were infected with B2c strain, and recombinant mouse IFN-λ2 or IFN-λ3 at 10 ng/mL and 1000 ng/mL were added to treat the RABV infected cells at 24 h post infection (hpi), and the virus titers, the levels of mRNA of RABV N gene (N mRNA) and viral RNA (vRNA) were measured at 24 and 48 h after the treatment. The gene discussed is IFNL2; the disease is infection.