The most negatively related genes included KRT19, ZFP36L1, AHNAK, IGFBP7, and TM4SF1; interestingly, KRT19, AHNAK2, and TM4SF1 were previously reported as promising diagnostic candidates and were upregulated in PDA-promoting malignancy (43–45). The gene discussed is TM4SF1; the disease is Patent ductus arteriosus.