PFKFB3 and neoplasm: This demonstrated that PFKFB3 and PFKP were overexpressed in tumour tissue, whilst there was modest upregulation of PFKFB2 and expression of PFKFB1 and PFKFB4 was downregulated, compared with healthy tissue from the same pancreatic resection (n = 39, p < 0.0001, Fig. 1a/b).