In the current studies, we also carried out c-Myc mRNA expression levels in normal, adjoining and tumor tissues, as c-Myc is one of the well-known targets of β-catenin/TCF4, and we found that the expression of c-Myc was increased in tumor tissues as compared to normal mucosa (data not shown). Yochum et al. [31] also showed, by using the human HCT116 cell line as a model, that TCF4/β-catenin complexes assembled at the MYC 3′ enhancer and coordinated a chromatin loop with the MYC proximal promoter to activate MYC expression, which is in accordance with our studies [31–33]. Here, MYC is linked to neoplasm.