To gain insight into potential function of such genes in CRC, we sequenced six colon cancer cell lines (HCT15, SW480, SW620, SW116, HT29, HCT116, Colo205) and two normal colon cell lines (CCD841, HCoEpiC) and found that all 8 cell lines (both tumor and normal) had low expression of these three ICP related genes, IFNγ and six classical ICPs compared to primary tumors and normal tissues in HCA and PCA analyses (Figures S2A,B). This evidence concerns the gene IFNG and malignant colon neoplasm.