In summary, by applying NGS to study the expression of six classical ICPs and their co-expression networks, we found not only the well-established connection between IFNγ and the expression of ICPs in CRC, a relatively immunotherapy-refractory tumor type, but also, a novel set of ICPRGs as well as potential new hub genes which may be potential therapeutic targets. The gene discussed is IFNG; the disease is neoplasm.