PRF1 and neoplasm: As for T cell related cytotoxins, essential in tumor killing, stacked FPKM of 8 genes (PRF1, GZMM, GZMK, GZMH, GZMB, GZMA, FASLG, and FAS) demonstrated that these genes were more highly expressed 1.7-fold (P = 0.037) in IFNγ positive tumors compared to IFNγ negative tumors (Figure S3A).