PPARGC1A and obesity due to melanocortin 4 receptor deficiency: To further investigate the effects of the lack of the MIF tautomerase activity on HFD-induced obesity, we detected the expressions of the genes related with lipid synthesis (Srebp-1c, Fans, SCD-1), lipolysis (ATGL, HSL, G0S2), fatty acid oxidation (PPARα, CPT-1α, ACOX1) and adiposeătissueăbrowning (UCP1, PGC-1α, adiponectin, Elov13, Dio2 and Prdm16) in white adipose tissues.