To test EDI in a heart failure model, we subjected C57BL/6 mice to TAC surgery to induce chronic pressure overload and applied an AAV9 vector encoding EDI or enhanced green fluorescent protein (AAV9-eGFP) under the control of the CMV-enhanced myosin light chain promotor for efficient cardiac expression (Supplementary Fig. 7A)21,33. This evidence concerns the gene MYH14 and heart failure.