HK2 and infection: Similarly to that of C. albicans, full virulence of C. neoformans also relies on both glycolysis and gluconeogenesis, depending on the infection model; C. neoformans’s virulence requires Pck1 in a murine tail vein infection model, whereas Hxk1/Hxk2 and Pyk1 are required for virulence in a murine inhalation model (13, 31).