We also observed the concurrent remission in pT-ION-induced allodynia and anxiety-like behaviors by inhibition of bilateral LHb through chemicogenetic methods or by overexpression of Tacr3. The present study shows that pT-ION-induced allodynia and anxiety-like behaviors can be differentially modulated by altering Tacr3 expression and subsequent LHb function. This evidence concerns the gene TACR3 and Anxiety.