Here we provide the first demonstration that selective inhibition of glutamatergic neurons in the unilateral LHb alleviates pT-ION-induced anxiety-like behaviors but not allodynia, while both allodynia and anxiety-like behaviors are significantly alleviated by bilateral chemicogenetic inhibition of LHb or Tacr3 overexpression, indicating that the LHb regulates allodynia and anxiety-like behaviors through different mechanisms. Here, TACR3 is linked to Anxiety.