To define the phenotype of the cells expressing KLF4 after cerebral ischemic stroke, dual-IF was performed on frozen sections of ischemic hemisphere taken from sham-operated mice (sham) or 2 and 7 days post-ischemia, using antibodies specific for KLF4 (AlexaFluor-488), the endothelial-specific marker CD31 (Cy-3) and astrocyte marker GFAP (Cy-3), or KLF4 (Cy-3) and microglial marker Mac-1 (AlexaFluor-488). The gene discussed is GFAP; the disease is ischemia.