To directly investigate the role and mechanism of KLF4 in regulating the cerebral vascular endothelial inflammation following CIS, we next employed KLF4-specific siRNA to knockdown the expression of KLF4 in bEnd3 cells and performed western blotting to examine the impact of this on the expression of E-selectin, VCAM-1, ICAM-1, and phosphorylation of NF-κB in response to OGD/R. The gene discussed is KLF4; the disease is in situ carcinoma.