Consistent with these results, in the current study, dual-IF staining showed that KLF4 expression co-localized with Claudin-5 on the blood vessels in the ischemic hemisphere at days 2 and 7 post-ischemia, indicating that KLF4 constitutively associated with Claudin5 following focal cerebral ischemia. This evidence concerns the gene CLDN5 and ischemia.