The observed amplifications complied with clinical specifications and additional molecular findings [28–33]: we detected an EGFR amplification in four samples (i.e. three EGFR-mutated lung tumors and one glioblastoma specimen), a BRAF amplification in two samples (both BRAF-mutated melanoma samples), a PIK3CA amplification in one sample (EGFR-mutated NSCLC case that showed progression upon tyrosine kinase inhibitor treatment), a KRAS amplification in one sample (NSCLC case without any identified mutations), and an ERBB2 (HER2) amplification in one sample (salivary duct carcinoma). This evidence concerns the gene PIK3CA and salivary duct carcinoma.