MSH6 and prostate carcinoma: In three positive control samples (two endometrial tumors and the MSH6-deficient prostate carcinoma) none of the microsatellites included in the pentaplex PCR assay was classified as unstable by the smMIP-based NGS analysis, suggesting the sensitivity for these individual loci is lower than the pentaplex PCR due to the objective cut-off (mean number of alleles + (2 x SD)).