The current study assessed in the order of the coding sequences and flanking sequences of five genes: COMP (exons 8–19), MATN3 (exon 2), COL9A2 (exon 3), COL9A3 (exon 3), COL9A1 (exon 8) associated with MED by Sanger sequencing, and characterized the associated clinical features. This evidence concerns the gene MATN3 and multiple epiphyseal dysplasia.