In epididymal adipose tissue, HF feeding significantly increased the gene expression of the pro-inflammatory marker, monocyte chemoattractant protein-1 (MCP-1; LF vs. HF, p < 0.05; Figure 5C) and this was significantly reduced by 10% 2′-FL supplementation (2′-FL vs. HF, p < 0.05). Here, CCL2 is linked to hydrops fetalis.