Further, the hybrid N-glycan attached to Kv3.1b expressed in the HuNB(-MGAT2) cell line, as well as the rat NB_1(-Mgat2) cell line [25], had the α1–3 mannose removed (or less than 4 mannose residues) [38] since it was insensitive to Endo H. Additionally, changes in the N-glycosylation pathway due to silencing MGAT2 could be partially recovered by transient transfection with mouse MGAT2, as transfection efficiency is low in NB cells. The gene discussed is MGAT2; the disease is neuroblastoma.