LEP and breast carcinoma: The increase in both ObRl and ObRsh was then confirmed by evaluating protein levels using immunoblotting in AnaR compared to MCF-7 aro cells (Figure 2b), whereas ELISA measurement in breast cancer cell-derived conditioned media showed that AnaR cells exhibited a 2.5 fold higher leptin secretion than MCF-7 aro cells (Figure 2c).