To better understand the role of ECs and NAEs in bladder cancer, we analyzed gene expression data from TGCA database regarding the metabolic pathway of the ECS, comprising the synthetic enzymes (PLCB1-4, PTPN22, ABHD4, GDE1, DAGLA, DAGLB, and NAPE-PLD) and the hydrolytic ones (FAAH, NAAA, and MGLL). This evidence concerns the gene PLCB1 and urinary bladder cancer.