Our analysis predicted several potential SIX1-targeting miRNAs, among which only miR-489-3p and the positive control miR-548a-3p, which has been shown to inhibit SIX1 expression in breast and liver cancer cells,8 could inhibit SIX1 expression in 293T cells by western blot analysis (Figure 1A). The gene discussed is SIX1; the disease is liver cancer.