In addition, the expression of Slug and Vimentin, the markers of epithelial-to-mesenchymal transition (EMT) involved in cancer metastasis, was downregulated by miR-489 overexpression or SIX1 knockdown, suggesting that the miR-489/SIX1 axis may play a role in metastasis (Figure 4D). The gene discussed is VIM; the disease is cancer.