However, the 1.5% ISPOC significantly reduced the number of positive apoptotic cells in the hippocampus CA1 region (P < 0.05), whereas when pretreated with Ro318220 (the inhibitor of p-Cx43) before reperfusion, apoptotic cells induced by ischemia increased clearly (P < 0.01). This evidence concerns the gene GJA1 and ischemia.