Quantitative PCR and western blots showed that MACROD2 expression was decreased in all six HCC cell lines, especially those with high metastatic potential (MHCC97L, MHCC97H, and HCCLM3), compared with that in the non-cancerous hepatic cell line L0-2 (Fig. 1f). The gene discussed is MACROD2; the disease is hepatocellular carcinoma.