To determine the biological effects of MACROD2 expression in HCC cells, we used short hairpin RNA (shRNA) to knock down MACROD2 in HepG2 and PLC/PRF/5 cells, which normally display high levels of MACROD2 expression, and we overexpressed MACROD2 in MHCC97H and HCCLM3 cells, which normally display low levels of MACROD2 expression. Here, MACROD2 is linked to hepatocellular carcinoma.