Klotho knockdown noticeably caused the increase of intervertebral disc injuries, judged by AF and NP cells (Figure 5(a)), TLR4 and p-IκBα accumulation, IκBα reductions (Figure 5(b)), and enhanced expressions of IL-1β, NOS2, and IL-18 (Figure 5(c)). The gene discussed is NOS2; the disease is atrial fibrillation.