KL and vertebral column disorder: The Klotho gene encodes a single transmembrane protein and secreted protein, which facilitates regulation of various cellular processes by affecting multiple cell membrane receptors and transporters and related signaling pathways such as aging, inflammation, apoptosis, oxidative stress, etc. Human Klotho gene polymorphisms are associated with pathophysiological bone loss in aging [15], spinal disease [16], osteocalcin levels [15], and bone mineral density [17].