At approximately 10 days after the inoculation, when the tumor size had reached 40 mm3, mice were divided into 4 groups and treated by IT in combination with saponin: (1) IT-Robo1 + saponin, (2) IT-Robo1 only, (3) saponin only, and (4) PBS control. This evidence concerns the gene ROBO1 and neoplasm.