We mapped the regional presentation score of the most highly represented protein in the neuroblastoma ligandome, NPY (29 MHC Class I peptides detected in 16 neuroblastomas), finding a highly significant concordance between the empirically detected peptides and those regions of the protein expected to be highly presented (Figure 3E; p = 0.000011), and find no peptides in the ligandome derived from the signal peptide region (aa 1–28) which is cleaved from the full-length pro-NPY protein. This evidence concerns the gene NPY and neuroblastoma.