Previously we showed that ICC collected by FACS, as performed in this study, showed minimal expression of Pdgfra (a marker for fibroblast-like cells), Myh11 (a marker for SMC) and Uchl1 (a marker for neurons) and elevated levels of Kit transcripts (ICC marker) relative to the unsorted cells, suggesting that sorted ICC were highly purified by FACS (Peri et al., 2013). The gene discussed is UCHL1; the disease is intrahepatic cholangiocarcinoma.