After we excluded some DEGs and DELs which have been reported in CRC, we finally chose the top 10 (CA2, CHP2, SLC51B, ITM2C, LRRC19, SULT1B1, SMPDL3A, MOGAT2, C1orf115 and RP11-396O20.2) as our candidate biomarkers for qRT-PCR validation in cell lines according to their differential expression levels. The gene discussed is CA2; the disease is colorectal carcinoma.