The Ki67-positive rates of the NT group (66.03% ± 9.21%) and KT group (39.53% ± 3.54%) were significantly higher than those of the NC group (3.30% ± 0.27%) and KC group (3.87% ± 0.41%), and the positive rate of the KT group was significantly reduced compared to that of the NT group (P < 0.001). The gene discussed is MKI67; the disease is keratoconus.