Compared with lower‐grade astrocytomas, GBMs express higher levels of AQP1, and are typified by enhanced proliferation, migration, and invasion; therefore, we evaluated these characteristics in vitro using the U251 and U87 clones that express exogenous levels of AQP1 and in their mock‐transfected controls. The gene discussed is AQP1; the disease is astrocytoma (excluding glioblastoma).