Next, to examine whether Lpiat1 specific depletion in hepatocytes causes steatosis, we generated hepatocyte-specific Lpiat1 knockout mice (LKO), by crossing the Lpiat1f/f mice with the albumin-Cre (Alb-Cre) mice.20 LPIAT1 was depleted in the liver in LKO mice as shown by western blotting (figure 1E). The gene discussed is ALB; the disease is steatosis.