Average MALDI MSI profiles of the defensins of each FFPE tumor biopsy is depicted in Figure 3B. In these average MALDI MSI spectra from nine patients responding and 16 patients nonresponding to immunotherapy treatment, an evaluation on the clear presence or absence of ions corresponding to neutrophil defensin 1, neutrophil defensin 2 and neutrophil defensin 3, was performed. Here, DEFA3 is linked to neoplasm.