NOTCH2 and Alzheimer disease: Previous studies showed that hN2 and Hsp90 co-precipitate with pTau in the brains of AD patients, and exhibit a synergistic effect on the attenuation of pTau pathology in cell models (Ali et al., 2016), Here, we used the single-molecule pull-down (SMPull) assay to identify the interplay between NMNAT, Hsp90, and pTau.