For this purpose, we generated stable pool cells with FBXO22 depletion, either by lentiviruses encoding CAS9 and a guide RNA targeting FBXO22 gene locus (gFBXO22) with gNS as a non-specific control (Fig. 3a), or by retroviruses encoding FBXO22-specific shRNA (shFBXO22) with shNC as a negative control (Fig. 3b), in two colorectal cancer cell lines, SW620 and SW480. Here, FBXO22 is linked to colorectal cancer.