We initially validated the location and expression of cdc6 in different cell types with in situ hybridization and observed that Cdc6 mRNA mainly located in the cytoplasm, and its signal was very clear in tumor cells and also was much stronger than that in normal PBMCs (Figure 2).These indicate a distinct expression pattern between tumor cells and PBMCs, which further confirmed our view of point. This evidence concerns the gene CDC6 and neoplasm.