Of the two tests employed, all (100%) samples were negative for Yellow Fever, Dengue Fever, West Nile, Chikungunya and Rift Valley Fever viruses by IgM ELISA; Flavi, Alpha, Orthobunya, Yellow Fever, West Nile, Dengue, Rift Valley Fever and Chikungunya viruses by PCR; Rickettsia spp., Leptospira spp. and Salmonella spp. The gene discussed is CD40LG; the disease is dengue disease.