Additionally, Th17 cells (CD3 + CD4 + IL-17+) were significantly higher (p < 0.001) in the ALS group and T regulatory (Treg) cells (CD3 + CD4 + CD25 + FoxP3+) were significantly lower (p < 0.05) in comparison to controls. This evidence concerns the gene CD4 and amyotrophic lateral sclerosis.