To elucidate whether METTL3-induced CRC glycolysis depends on its methyltransferase function, we constructed METTL3 wild-type (pcDNA3.1-METTL3) and mutant (pcDNA3.1-METTL3-mut, with MTase domain deletion) recombination plasmids (Figure S2g) [28]. The gene discussed is METTL3; the disease is colorectal carcinoma.