To elucidate whether METTL3 plays a role in CRC glycolysis and tumorigenesis, we then performed RNA-seq analysis to compare the gene expression profiles of METTL3-knockout and wild type (WT) HCT116 CRC cells, which exhibit higher METTL3 expression (Figure S1a). This evidence concerns the gene METTL3 and colorectal carcinoma.