Therefore, in the current study, hydatid cyst samples collected from human patients with echinococcosis and stored as formalin-fixed paraffin-embedded (FFPE) tissues were subjected to sequence analysis by analyzing mitochondrial cytochrome c oxidase subunit 1 (cox1), cytochrome b (cytb), and NADH subunit 1 (nad1) genes, to investigate the possible genetic diversity in the hydatid cyst samples in Pakistan. Here, MT-CO1 is linked to echinococcosis.