ACAN and atrial fibrillation: For ACAN, with an FC of 4.32 in native tissues and an FC of −2.35 in monolayer cells, the corresponding signals decreased in mild and severe degenerated samples in monolayer cells compared to native tissue as mentioned above (from 6901.30 (mild) and 2063.57 (severe) in native AF to 161.73 (mild) and 410.97 (severe) in monolayer).