Hedgehog signals are involved in regulating the nuclear translocation of GLI-1, which triggers the transcription of target genes, including MYC. Combination therapy with hedgehog-inhibiting agents such as AY9944, GANT61 and 5-FU, irinotecan, or oxaliplatin, decreased cell viability of CRC organoids compared to single treatment [138]. This evidence concerns the gene MYC and colorectal carcinoma.