The genera Clostridium XI, Clostridium IV and Escherichia/Shigella, the biomarkers of the HFD group based on the LEfSe analysis, were found to be positively correlated with the proinflammatory cytokine TNF-α, indicating that these genera may have a proinflammatory role during obesity development. This evidence concerns the gene TNF and obesity due to melanocortin 4 receptor deficiency.