To study the PA2G4P4 RNA localization in BICa samples, in situ hybridization was performed using a digoxigenin-labelled PA2G4P4 RNA antisense probe (Figure 1) on both tumor samples, divided into low( LG) and high (HG) grade BlCa samples, and on adjacent bladder tissues (Figure 3). The gene discussed is PA2G4P4; the disease is bladder transitional cell carcinoma.