To examine the effect of Calebin A and/or 5-FU on TNF-β-promoted cell viability and proliferation of CRC cells (HCT116 and HCT116R) in the monolayer of the inflammatory tumor environment, the cells were treated as described in detail in the Materials and Methods section and the capacity of proliferation was assessed by MTT assay. The gene discussed is LTA; the disease is colorectal carcinoma.