LTA and neoplasm: To examine the differential activity of the Calebin A, we next evaluated whether Calebin A and/or 5-FU inhibited the capacity of two CRC cell lines (parental HCT116 and chemoresistant HCT116R) for colonosphere formation (Figure 2A–C) and to suppress migration (Figure 2D–F) in TNF-β-induced tumor environments using phase-contrast light microscopy.