The co-treatment with Calebin A and 5-FU and/or with TNF-β showed synergistic enhancement in promoting caspase-3 cleavage in both CRC cells (Figure 5A,B) compared to control tumor cultures, indicating that Calebin A enhanced TNF-β/5-FU-promoted caspase-3 dependent apoptosis in CRC cells. This evidence concerns the gene CASP3 and neoplasm.