To determine signal transduction pathways participating in the anti-inflammatory actions of Calebin A, we examined transcription factor NF-κB linked with malignity and survival of CRC cells (HCT116, HCT116R) and performed immunofluorescence labeling for p65-NF-κB as described in the Materials and Methods section. The gene discussed is NFKB1; the disease is colorectal carcinoma.