ACTA1 and pulmonary fibrosis: The body weights (Supplementary Figure 1A), both hematoxylin and eosin (H&E) and Masson-trichrome staining of lung tissues (Supplementary Figure 1B), and immunofluorescence staining of collagen I, fibronectin, and α-smooth muscle actin (α-SMA) in the lungs (Supplementary Figure 1C) all confirmed that bleomycin caused severe pulmonary fibrosis.