TNF and gingivitis: Currently biomarkers have also been used in the diagnosis of gingivitis and inflammatory changes whose etiology is due to the presence of non-removable orthodontic devices.[3] Inflammatory cytokines from crevicular fluid and saliva have been evaluated, the evaluation of IL-6, IL-1β, IL-8, TNF-α and IL-10 cytokines in the unstimulated saliva and crevicular fluid by ELISA will be performed at baseline (T0), 7 (T1) and 21 (T2) days after treatment; we have included cytokines with an inflammatory and regulatory profile (IL-10).