For myotubes, DUX4 expression did not represent a significant classifier of FSHD status (FSHD vs control: Wilcoxon P = 0.11, AUC = 0.583, n = 33 (18 FSHD, 15 control), Fig. 2I). Here, DUX4 is linked to facioscapulohumeral muscular dystrophy.