DUX4 and facioscapulohumeral muscular dystrophy: early (8 h) DUX4 target gene expression signature was not a significant classifier of FSHD status (FSHD vs control: Wilcoxon P = 0.66, AUC = 0.548, n = 33 (18 FSHD, 15 control), Fig. 2E and F).